Identification of Residues in β-Lactamase Critical for Binding β-Lactamase Inhibitory Protein
نویسندگان
چکیده
منابع مشابه
Fine Mapping of the Sequence Requirements for Binding of β-Lactamase Inhibitory Protein (BLIP) to TEM-1 β-Lactamase Using a Genetic Screen for BLIP Function
0022-2836/$ see front matter © 2009 E β-Lactamase inhibitory protein (BLIP) binds and inhibits a diverse collection of class A β-lactamases with a wide range of affinities. Alaninescanning mutagenesis was previously performed to identify the amino acid sequence requirements of BLIP for binding the TEM-1, SME-1, SHV-1, and Bla1 β-lactamases. Twenty-three BLIP residues that contact TEM-1 βlactama...
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β-Lactamase residues in milk represent a public health risk. The cylinder plate detection method, which is based on bacterial growth, is laborious and time consuming. In this study, 15 monoclonal antibodies (mAbs) were selected against Temoneira (TEM) 1 β-lactamase. A sandwich enzyme-linked immunosorbent assay (ELISA) based on an optimum mAb pair was developed and validated for the detection of...
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Author affiliations: Justus Liebig University, Giessen, Germany (L. Falgenhauer, S.-E. Waezsada, K. Gwozdzinski, H. Ghosh, S. Doijad, C. Imirzalioglu, A. Goesmann, T. Chakraborty); German Center for Infection Research, Giessen, Germany (L. Falgenhauer, S.-E. Waezsada, K. Gwozdzinski, H. Ghosh, S. Doijad, C. Imirzalioglu, T. Chakraborty); Leibniz Institute DSMZ—German Collection of Microorganism...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1999
ISSN: 0021-9258
DOI: 10.1074/jbc.274.11.6963